3M31

Structure of the C150A/C295A mutant of S. cerevisiae Ero1p

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Endoplasmic oxidoreductin-1	polymer	388	44730.3	1	UniProt (Q03103) Pfam (PF04137) In PDB	Saccharomyces cerevisiae (yeast)	Ero1p, Endoplasmic oxidoreductase protein 1
2	A	1-ETHYL-PYRROLIDINE-2,5-DIONE	non-polymer		127.1	1	Chemie (NEN)
3	A	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
4	A	CADMIUM ION	non-polymer		112.4	1	Chemie (CD)
5		water	water		18.0	201	Chemie (HOH)

Sequence modifications

A: 56 - 424 (UniProt: Q03103)

PDB	External Database	Details
Ser 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
Ser 47	-	expression tag
Ser 48	-	expression tag
Gly 49	-	expression tag
Leu 50	-	expression tag
Val 51	-	expression tag
Pro 52	-	expression tag
Arg 53	-	expression tag
Gly 54	-	expression tag
Ser 55	-	expression tag
Ala 150	Cys 150	engineered mutation
Ala 295	Cys 295	engineered mutation
Leu 425	-	expression tag
Glu 426	-	expression tag
Arg 427	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	44730.3
Non-Polymers*	Number of molecules	3
	Total formula weight	1025.1
All*	Total formula weight	45755.4

*Water molecules are not included.