3M00
Crystal Structure of 5-epi-aristolochene synthase M4 mutant complexed with (2-cis,6-trans)-2-fluorofarnesyl diphosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Aristolochene synthase | polymer | 550 | 63209.8 | 1 | UniProt (Q40577) Pfam (PF01397) Pfam (PF03936) | Nicotiana tabacum (American tobacco tobacco) | 5-epi-aristolochene synthase, EAS |
| 2 | B (A) | (2E,6E)-2-fluoro-3,7,11-trimethyldodeca-2,6,10-trien-1-yl trihydrogen diphosphate | non-polymer | 400.3 | 1 | Chemie (2CF) | |||
| 3 | C, D, E (A) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 4 | F (A) | water | water | 18.0 | 372 | Chemie (HOH) |
Sequence modifications
A: 1 - 548 (UniProt: Q40577)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Thr 274 | Ala 274 | engineered mutation |
| Ile 372 | Val 372 | engineered mutation |
| Leu 406 | Tyr 406 | engineered mutation |
| Ile 516 | Val 516 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 63209.8 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 473.2 | |
| All* | Total formula weight | 63683.0 |
