3LXV
Tyrosine 447 of Protocatechuate 3,4-Dioxygenase Controls Efficient Progress Through Catalysis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E (A, B, C) | Protocatechuate 3,4-dioxygenase alpha chain | polymer | 200 | 22278.8 | 3 | UniProt (P00436) Pfam (PF00775) | Pseudomonas putida | |
| 2 | B, D, F (M, N, O) | Protocatechuate 3,4-dioxygenase beta chain | polymer | 238 | 26671.3 | 3 | UniProt (P00437) Pfam (PF12391) Pfam (PF00775) | Pseudomonas putida | |
| 3 | G, IA, V (A, C, B) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 4 | AA, BA, H, JA, N... (N, A, C, M) | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) | |||
| 5 | CA, EA, I, J, KA... (N, A, C, O, M...) | BETA-MERCAPTOETHANOL | non-polymer | 78.1 | 15 | Chemie (BME) | |||
| 6 | K, SA, X (A, O, B) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 3 | Chemie (TRS) | |||
| 7 | GA, L, MA, S, T (N, A, C, M) | CARBONATE ION | non-polymer | 60.0 | 5 | Chemie (CO3) | |||
| 8 | DA, M, VA (N, M, O) | FE (III) ION | non-polymer | 55.8 | 3 | Chemie (FE) | |||
| 9 | FA, U, UA, WA (N, M, O) | 4-NITROCATECHOL | non-polymer | 155.1 | 4 | Chemie (4NC) | |||
| 10 | HA, TA, Y (N, O, B) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 11 | AB, BB, CB, XA, YA... (N, C, O, A, M...) | water | water | 18.0 | 1062 | Chemie (HOH) |
Sequence modifications
M, N, O: 301 - 538 (UniProt: P00437)
| PDB | External Database | Details |
|---|---|---|
| His 447 | Tyr 148 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 146850.2 | |
| Non-Polymers* | Number of molecules | 43 |
| Total formula weight | 3665.6 | |
| All* | Total formula weight | 150515.9 |
