3LX4

Stepwise [FeFe]-hydrogenase H-cluster assembly revealed in the structure of HydA(deltaEFG)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Fe-hydrogenase	polymer	457	49366.1	2	UniProt (Q9FYU1) Pfam (PF02906) Pfam (PF02256)	Chlamydomonas reinhardtii	Iron hydrogenase, Iron-hydrogenase HydA1
2	C, F (A, B)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
3	D, G (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
4	E, H (A, B)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
5	I, J (A, B)	water	water		18.0	775	Chemie (HOH)

Sequence modifications

A, B: 17 - 457 (UniProt: Q9FYU1)

PDB	External Database	Details
Met 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Gln 12	-	expression tag
Asp 13	-	expression tag
Pro 14	-	expression tag
Asn 15	-	expression tag
Ser 16	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	98732.2
Non-Polymers*	Number of molecules	6
	Total formula weight	892.3
All*	Total formula weight	99624.5

*Water molecules are not included.