3LRK
Structure of alfa-galactosidase (MEL1) from Saccharomyces cerevisiae
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Alpha-galactosidase 1 | polymer | 479 | 53127.6 | 1 | UniProt (P04824) Pfam (PF16499) Pfam (PF17801) In PDB | Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast) | Alpha-D-galactoside galactohydrolase 1, Melibiase 1 |
| 2 | B | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G79924RM) | |||
| 3 | C | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 4 | A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 4 | Chemie (NAG) | |||
| 5 | A | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
| 6 | A | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 7 | A | 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 209.2 | 1 | Chemie (BTB) | |||
| 8 | water | water | 18.0 | 434 | Chemie (HOH) |
Sequence modifications
A: 1 - 471 (UniProt: P04824)
| PDB | External Database | Details |
|---|---|---|
| Val 181 | Ile 181 | engineered mutation |
| Asp 472 | - | expression tag |
| Tyr 473 | - | expression tag |
| Lys 474 | - | expression tag |
| Asp 475 | - | expression tag |
| Asp 476 | - | expression tag |
| Asp 477 | - | expression tag |
| Asp 478 | - | expression tag |
| Lys 479 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 53127.6 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 848.8 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1393.3 | |
| All* | Total formula weight | 55369.8 |
