3LQA

Crystal structure of clade C gp120 in complex with sCD4 and 21c Fab

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (C)	T-cell surface glycoprotein CD4	polymer	192	21472.3	1	UniProt (P01730) Pfam (PF00047) Pfam (PF05790)	Homo sapiens
2	B (G)	Envelope glycoprotein gp160	polymer	332	36794.6	1	UniProt (Q1PHM6) Pfam (PF00516)	HIV-1
3	C (H)	Heavy chain of anti HIV Fab from human 21c antibody	polymer	231	24619.6	1		Homo sapiens
4	D (L)	Light chain of anti HIV Fab from human 21c antibody	polymer	217	22815.3	1		Homo sapiens
5	E, F, G, H (G)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)

Sequence modifications

C: 1 - 182 (UniProt: P01730)

PDB	External Database	Details
Ile 183	-	expression tag
Asp 184	-	expression tag
Gly 185	-	expression tag
Arg 186	-	expression tag
His 187	-	expression tag
His 188	-	expression tag
His 189	-	expression tag
His 190	-	expression tag
His 191	-	expression tag
His 192	-	expression tag

G: 82 - 293 (UniProt: Q1PHM6)

PDB	External Database	Details
Ile 89	Thr 89	engineered mutation
Asp 226	Asn 226	engineered mutation
Ile 232	Thr 232	engineered mutation
Thr 285	Asn 285	engineered mutation

G: 325 - 494 (UniProt: Q1PHM6)

PDB	External Database	Details
Gly 294	-	linker
Ala 323	-	linker
Gly 324	-	linker
Asn 329	Ser 329	engineered mutation
Ile 388	Thr 388	engineered mutation
Asp 447	Asn 447	engineered mutation
Ser 495	-	expression tag
Gly 496	-	expression tag
His 497	-	expression tag
His 498	-	expression tag
His 499	-	expression tag
His 500	-	expression tag
His 501	-	expression tag
His 502	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	105701.8
Non-Polymers*	Number of molecules	4
	Total formula weight	884.8
All*	Total formula weight	106586.6

*Water molecules are not included.