3LMX
Tyrosine 447 of Protocatechuate 34,-Dioxygenase Controls Efficient Progress Through Catalysis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E (A, B, C) | Protocatechuate 3,4-dioxygenase alpha chain | polymer | 200 | 22278.8 | 3 | UniProt (P00436) Pfam (PF00775) | Pseudomonas putida | 3,4-PCD |
| 2 | B, D, F (M, N, O) | Protocatechuate 3,4-dioxygenase beta chain | polymer | 238 | 26671.3 | 3 | UniProt (P00437) Pfam (PF12391) Pfam (PF00775) | Pseudomonas putida | 3,4-PCD |
| 3 | FA, G, JB, LA, MA... (B, A, O, N, M) | GLYCEROL | non-polymer | 92.1 | 10 | Chemie (GOL) | |||
| 4 | FB, GA, H, I, NA... (O, B, A, N, M...) | SULFATE ION | non-polymer | 96.1 | 11 | Chemie (SO4) | |||
| 5 | BA, BB, CA, DA, EA... (M, C, O, B, A...) | BETA-MERCAPTOETHANOL | non-polymer | 78.1 | 23 | Chemie (BME) | |||
| 6 | AA, AB, IB, L, SA... (M, C, O, A, N) | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
| 7 | CB, IA, O (O, N, M) | FE (III) ION | non-polymer | 55.8 | 3 | Chemie (FE) | |||
| 8 | DB, JA, KA, P, Q... (O, N, M) | 3,4-DIHYDROXYBENZOIC ACID | non-polymer | 154.1 | 6 | Chemie (DHB) | |||
| 9 | NB, OB, PB, QB, RB... (A, M, B, N, C...) | water | water | 18.0 | 600 | Chemie (HOH) |
Sequence modifications
M, N, O: 301 - 538 (UniProt: P00437)
| PDB | External Database | Details |
|---|---|---|
| His 447 | Tyr 148 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 146850.2 | |
| Non-Polymers* | Number of molecules | 59 |
| Total formula weight | 5079.7 | |
| All* | Total formula weight | 151929.9 |
