3LM8
Crystal Structure of Thiamine pyrophosphokinase from Bacillus subtilis, Northeast Structural Genomics Consortium Target SR677
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Thiamine pyrophosphokinase | polymer | 222 | 25367.4 | 4 | UniProt (O34664) Pfam (PF04263) Pfam (PF04265) | Bacillus subtilis | TPK, Thiamine diphosphokinase |
| 2 | E, I, O, Q (A, B, C, D) | 3-(4-AMINO-2-METHYL-PYRIMIDIN-5-YLMETHYL)-5-(2-HYDROXY-ETHYL)-4-METHYL-THIAZOL-3-IUM | non-polymer | 265.4 | 4 | Chemie (VIB) | |||
| 3 | F, J, K, P (A, B, C) | PHOSPHATE ION | non-polymer | 95.0 | 4 | Chemie (PO4) | |||
| 4 | G, H, L, M, N (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 5 | R, S, T, U (A, B, C, D) | water | water | 18.0 | 67 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 214 (UniProt: O34664)
| PDB | External Database | Details |
|---|---|---|
| Val 203 | Ala 203 | engineered mutation |
| Leu 215 | - | expression tag |
| Glu 216 | - | expression tag |
| His 217 | - | expression tag |
| His 218 | - | expression tag |
| His 219 | - | expression tag |
| His 220 | - | expression tag |
| His 221 | - | expression tag |
| His 222 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 101469.8 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 1562.8 | |
| All* | Total formula weight | 103032.6 |
