3LES

2F5 Epitope scaffold ES2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	RNA polymerase sigma factor	polymer	179	20209.3	2	UniProt (Q9EZJ8)	Thermus aquaticus
2	C, D, E, F (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	G, H (A, B)	water	water		18.0	28	Chemie (HOH)

Sequence modifications

A, B: 93 - 271 (UniProt: Q9EZJ8)

PDB	External Database	Details
Glu 105	Gln 105	engineered mutation
Leu 107	Pro 107	engineered mutation
Glu 108	Leu 108	engineered mutation
Asp 110	Thr 110	engineered mutation
Lys 111	Leu 111	engineered mutation
Trp 112	Glu 112	engineered mutation
Ala 113	Glu 113	engineered mutation
Leu 115	Ile 115	engineered mutation
Gly 116	Asp 116	engineered mutation
Ala 117	Leu 117	engineered mutation
Ala 119	Arg 119	engineered mutation
Ala 155	Arg 155	engineered mutation
Gly 247	Arg 247	engineered mutation
Ala 249	Lys 249	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	40418.6
Non-Polymers*	Number of molecules	4
	Total formula weight	384.3
All*	Total formula weight	40802.9

*Water molecules are not included.