3LCW

L-KDO aldolase complexed with hydroxypyruvate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	N-acetylneuraminate lyase	polymer	319	35133.3	4	UniProt (P0A6L4) Pfam (PF00701) In PDB	Escherichia coli	L-KDO aldolase, N-acetylneuraminic acid aldolase, N-acetylneuraminate pyruvate-lyase, Sialic acid lyase, Sialate lyase, Sialic acid aldolase, NALase
2	A, B, C, D	3-HYDROXYPYRUVIC ACID	non-polymer		104.1	4	Chemie (3PY)
3	A, B, C, D	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
4		water	water		18.0	493	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 297 (UniProt: P0A6L4)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
His -6	-	expression tag
Ile -5	-	expression tag
Glu -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Ala 60	Glu 60	engineered mutation
His 98	Tyr 98	engineered mutation
Leu 115	Phe 115	engineered mutation
Gly 150	Asp 150	engineered mutation
Tyr 153	Asn 153	engineered mutation
Ile 251	Val 251	engineered mutation
Ile 265	Val 265	engineered mutation
Cys 281	Tyr 281	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	140533.1
Non-Polymers*	Number of molecules	8
	Total formula weight	800.5
All*	Total formula weight	141333.6

*Water molecules are not included.