3LCL
The D-sialic acid aldolase mutant V251I/V265I
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | N-acetylneuraminate lyase | polymer | 319 | 35319.3 | 4 | UniProt (P0A6L4) Pfam (PF00701) In PDB | Escherichia coli | N-acetylneuraminic acid aldolase, N-acetylneuraminate pyruvate-lyase, Sialic acid lyase, Sialate lyase, Sialic acid aldolase, NALase |
2 | A, B, C, D | SULFATE ION | non-polymer | 96.1 | 4 | Chemie (SO4) | |||
3 | water | water | 18.0 | 1218 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 297 (UniProt: P0A6L4)
PDB | External Database | Details |
---|---|---|
Met -21 | - | expression tag |
Gly -20 | - | expression tag |
His -19 | - | expression tag |
His -18 | - | expression tag |
His -17 | - | expression tag |
His -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
His -6 | - | expression tag |
Ile -5 | - | expression tag |
Glu -4 | - | expression tag |
Gly -3 | - | expression tag |
Arg -2 | - | expression tag |
His -1 | - | expression tag |
Met 0 | - | expression tag |
Ile 251 | Val 251 | engineered mutation |
Ile 265 | Val 265 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 141277.4 | |
Non-Polymers* | Number of molecules | 4 |
Total formula weight | 384.3 | |
All* | Total formula weight | 141661.6 |