3LCL

The D-sialic acid aldolase mutant V251I/V265I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	N-acetylneuraminate lyase	polymer	319	35319.3	4	UniProt (P0A6L4) Pfam (PF00701)	Escherichia coli	N-acetylneuraminic acid aldolase, N-acetylneuraminate pyruvate-lyase, Sialic acid lyase, Sialate lyase, Sialic acid aldolase, NALase
2	E, F, G, H (A, B, C, D)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
3	I, J, K, L (A, B, C, D)	water	water		18.0	1218	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 297 (UniProt: P0A6L4)

PDB	External Database	Details
Met -21	-	expression tag
Gly -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
His -6	-	expression tag
Ile -5	-	expression tag
Glu -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Ile 251	Val 251	engineered mutation
Ile 265	Val 265	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	141277.4
Non-Polymers*	Number of molecules	4
	Total formula weight	384.3
All*	Total formula weight	141661.6

*Water molecules are not included.