3LB8

Crystal structure of the covalent putidaredoxin reductase-putidaredoxin complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Putidaredoxin reductase	polymer	436	47265.7	2	UniProt (P16640) Pfam (PF07992) Pfam (PF14759)	Pseudomonas putida
2	C, D (C, D)	Putidaredoxin	polymer	106	11396.8	2	UniProt (P00259) Pfam (PF00111)	Pseudomonas putida	PDX
3	E, F (A, B)	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
4	G, H (C, D)	FE2/S2 (INORGANIC) CLUSTER	non-polymer		175.8	2	Chemie (FES)
5	I, J, K (A, B, C)	water	water		18.0	62	Chemie (HOH)

Sequence modifications

A, B: 2 - 422 (UniProt: P16640)

PDB	External Database	Details
Lys 423	-	expression tag
Ala 424	-	expression tag
Glu 425	-	expression tag
Leu 426	-	expression tag
Ser 427	-	expression tag
Ser 428	-	expression tag
Ala 429	-	expression tag
Pro 430	-	expression tag
Arg 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag

C, D: 1 - 106 (UniProt: P00259)

PDB	External Database	Details
Ser 73	Cys 74	engineered mutation
Ser 85	Cys 86	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	117324.9
Non-Polymers*	Number of molecules	4
	Total formula weight	1922.7
All*	Total formula weight	119247.7

*Water molecules are not included.