3L7K

Structure of the Wall Teichoic Acid Polymerase TagF, H444N + CDPG (15 minute soak)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Teichoic acid biosynthesis protein F	polymer	729	87011.1	4	UniProt (Q5HLM5) Pfam (PF00535) Pfam (PF04464) In PDB	Staphylococcus epidermidis
2	A, D, B, C	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
3	C, D, A, B	CHLORIDE ION	non-polymer		35.5	22	Chemie (CL)
4	D, A	{[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID	non-polymer		292.2	2	Chemie (EDT)
5	D	[CYTIDINE-5'-PHOSPHATE] GLYCERYLPHOSPHORIC ACID ESTER	non-polymer		477.3	1	Chemie (C2G)
6	D	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7		water	water		18.0	8	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 721 (UniProt: Q5HLM5)

PDB	External Database	Details
Asn 444	His 444	engineered mutation
Leu 722	-	expression tag
Glu 723	-	expression tag
His 724	-	expression tag
His 725	-	expression tag
His 726	-	expression tag
His 727	-	expression tag
His 728	-	expression tag
His 729	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	348044.4
Non-Polymers*	Number of molecules	32
	Total formula weight	2480.2
All*	Total formula weight	350524.6

*Water molecules are not included.