3L7K

Structure of the Wall Teichoic Acid Polymerase TagF, H444N + CDPG (15 minute soak)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Teichoic acid biosynthesis protein F	polymer	729	87011.1	4	UniProt (Q5HLM5) Pfam (PF00535) Pfam (PF04464)	Staphylococcus epidermidis
2	E, F, FA, G, R... (A, D, B, C)	SULFATE ION	non-polymer		96.1	6	Chemie (SO4)
3	AA, BA, CA, DA, GA... (C, D, A, B)	CHLORIDE ION	non-polymer		35.5	22	Chemie (CL)
4	JA, N (D, A)	{[-(BIS-CARBOXYMETHYL-AMINO)-ETHYL]-CARBOXYMETHYL-AMINO}-ACETIC ACID	non-polymer		292.2	2	Chemie (EDT)
5	EA (D)	[CYTIDINE-5'-PHOSPHATE] GLYCERYLPHOSPHORIC ACID ESTER	non-polymer		477.3	1	Chemie (C2G)
6	IA (D)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
7	KA, LA, MA, NA (A, B, C, D)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 721 (UniProt: Q5HLM5)

PDB	External Database	Details
Asn 444	His 444	engineered mutation
Leu 722	-	expression tag
Glu 723	-	expression tag
His 724	-	expression tag
His 725	-	expression tag
His 726	-	expression tag
His 727	-	expression tag
His 728	-	expression tag
His 729	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	348044.4
Non-Polymers*	Number of molecules	32
	Total formula weight	2480.2
All*	Total formula weight	350524.6

*Water molecules are not included.