3L2O

Structure-Based Mechanism of Dimerization-Dependent Ubiquitination by the SCFFbx4 Ubiquitin Ligase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	S-phase kinase-associated protein 1	polymer	149	16827.1	1	UniProt (P63208) Pfam (PF03931) Pfam (PF01466)	Homo sapiens (human)	Cyclin A/CDK2-associated protein p19, p19skp1, p19A, RNA polymerase II elongation factor-like protein, Organ of Corti protein 2, OCP-2, OCP-II, Transcription elongation factor B, SIII
2	B (B)	F-box only protein 4	polymer	312	35848.7	1	UniProt (Q9UKT5) Pfam (PF00646)	Homo sapiens (human)
3	C, D (A, B)	water	water		18.0	76	Chemie (HOH)

Sequence modifications

A: 1001 - 1163 (UniProt: P63208)

PDB	External Database	Details
Ala 1002	Pro 2	engineered mutation
-	Asp 38	deletion
-	Asp 39	deletion
-	Glu 40	deletion
-	Gly 41	deletion
-	Asp 42	deletion
-	Asp 43	deletion
-	Pro 70	deletion
-	Pro 71	deletion
-	Pro 72	deletion
-	Glu 73	deletion
-	Asp 74	deletion
-	Asp 75	deletion
-	Glu 76	deletion
-	Asn 77	deletion
-	Lys 78	deletion
-	Glu 79	deletion
-	Lys 80	deletion
-	Arg 81	deletion
Gly 1078	-	insertion
Gly 1079	-	insertion
Ser 1080	-	insertion
Gly 1081	-	insertion

B: 55 - 387 (UniProt: Q9UKT5)

PDB	External Database	Details
-	Thr 150	deletion
-	Arg 151	deletion
-	Arg 152	deletion
-	Ala 153	deletion
-	Ser 154	deletion
-	Lys 155	deletion
-	Ser 156	deletion
-	Ser 157	deletion
-	Arg 158	deletion
-	Pro 159	deletion
-	Met 160	deletion
-	Tyr 161	deletion
-	Gly 162	deletion
-	Ala 163	deletion
-	Val 164	deletion
-	Thr 165	deletion
-	Ser 166	deletion
-	Phe 167	deletion
-	Leu 168	deletion
-	His 169	deletion
-	Ser 170	deletion

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	52675.8
All*	Total formula weight	52675.8

*Water molecules are not included.