3KU9

X-ray structure of the mutant lys300met of polyamine oxidase from zea mays in complex with spermine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Polyamine oxidase	polymer	478	54529.3	2	UniProt (O64411) Pfam (PF01593) In PDB	Zea mays (maize)
2	C	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	A, B	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	2	Chemie (FAD)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	A, B	SPERMINE	non-polymer		202.3	2	Chemie (SPM)
6	B	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	B	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
8		water	water		18.0	53	Chemie (HOH)

Sequence modifications

A, B: 1 - 472 (UniProt: O64411)

PDB	External Database	Details
Met 300	Lys 328	engineered mutation
His 473	-	expression tag
His 474	-	expression tag
His 475	-	expression tag
His 476	-	expression tag
His 477	-	expression tag
His 478	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	109058.7
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	7
	Total formula weight	2328.5
All*	Total formula weight	111811.6

*Water molecules are not included.