3KSK

Crystal Structure of single chain PvuII

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Type-2 restriction enzyme PvuII	polymer	324	37692.8	2	UniProt (P23657) Pfam (PF09225)	Proteus hauseri	R.PvuII, Type II restriction enzyme PvuII, Endonuclease PvuII
2	C, D, F, G, H (A, B)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
3	E (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
4	I, J (B)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	2	Chemie (TRS)
5	K, L (A, B)	water	water		18.0	469	Chemie (HOH)

Sequence modifications

A, B: 2 - 157 (UniProt: P23657)

PDB	External Database	Details
Gly 318	-	expression tag
Ser 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag
His 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag

A, B: 162 - 317 (UniProt: P23657)

PDB	External Database	Details
Gly 158	-	linker
Ser 159	-	linker
Gly 160	-	linker
Gly 161	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	75385.6
Non-Polymers*	Number of molecules	8
	Total formula weight	842.8
All*	Total formula weight	76228.4

*Water molecules are not included.