3KRG
Structural insights into substrate specificity and the anti beta-elimination mechanism of pectate lyase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Pectate lyase | polymer | 399 | 43300.1 | 1 | UniProt (P39116) Pfam (PF00544) | Bacillus subtilis | PL |
| 2 | B (B) | alpha-D-galactopyranuronic acid-(1-4)-alpha-D-galactopyranuronic acid-(1-4)-alpha-D-galactopyranuronic acid-(1-4)-alpha-D-galactopyranuronic acid-(1-4)-alpha-D-galactopyranuronic acid-(1-4)-alpha-D-galactopyranuronic acid | branched | 1074.8 | 1 | In PDB GlyTouCan (G02254EM) | |||
| 3 | C (A) | COBALT (II) ION | non-polymer | 58.9 | 1 | Chemie (CO) | |||
| 4 | D (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | E (A) | water | water | 18.0 | 723 | Chemie (HOH) |
Sequence modifications
A: 1 - 399 (UniProt: P39116)
| PDB | External Database | Details |
|---|---|---|
| Ala 173 | Asp 194 | engineered mutation |
| Ala 180 | Asn 201 | engineered mutation |
| Ala 247 | Lys 268 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 43300.1 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 1074.8 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 151.0 | |
| All* | Total formula weight | 44525.8 |
