3KOT
Structure of the Citrobacter freundii effector binding domain containing three amino acid substitutions: T103V, S221A and Y264F
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | HTH-type transcriptional activator ampR | polymer | 219 | 24648.3 | 1 | UniProt (P12529) Pfam (PF03466) In PDB | Citrobacter freundii | |
2 | A | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
3 | water | water | 18.0 | 208 | Chemie (HOH) |
Sequence modifications
A: 83 - 291 (UniProt: P12529)
PDB | External Database | Details |
---|---|---|
Val 103 | Thr 103 | engineered mutation |
Thr 203 | Ala 203 | variant |
Val 204 | Ala 204 | variant |
Ala 221 | Ser 221 | engineered mutation |
Ala 230 | Gly 230 | variant |
Phe 264 | Tyr 264 | engineered mutation |
Thr 292 | - | expression tag |
Ser 293 | - | expression tag |
Gly 294 | - | expression tag |
Ser 295 | - | expression tag |
His 296 | - | expression tag |
His 297 | - | expression tag |
His 298 | - | expression tag |
His 299 | - | expression tag |
His 300 | - | expression tag |
His 301 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 24648.3 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 460.5 | |
All* | Total formula weight | 25108.7 |