3KOT

Structure of the Citrobacter freundii effector binding domain containing three amino acid substitutions: T103V, S221A and Y264F

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	HTH-type transcriptional activator ampR	polymer	219	24648.3	1	UniProt (P12529) Pfam (PF03466)	Citrobacter freundii
2	B, C, D, E, F (A)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
3	G (A)	water	water		18.0	208	Chemie (HOH)

Sequence modifications

A: 83 - 291 (UniProt: P12529)

PDB	External Database	Details
Val 103	Thr 103	engineered mutation
Thr 203	Ala 203	variant
Val 204	Ala 204	variant
Ala 221	Ser 221	engineered mutation
Ala 230	Gly 230	variant
Phe 264	Tyr 264	engineered mutation
Thr 292	-	expression tag
Ser 293	-	expression tag
Gly 294	-	expression tag
Ser 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag
His 301	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	24648.3
Non-Polymers*	Number of molecules	5
	Total formula weight	460.5
All*	Total formula weight	25108.7

*Water molecules are not included.