3KLH
Crystal structure of AZT-Resistant HIV-1 Reverse Transcriptase crosslinked to post-translocation AZTMP-Terminated DNA (COMPLEX P)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Reverse transcriptase/ribonuclease H | polymer | 564 | 64988.6 | 1 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) | Human immunodeficiency virus type 1 (HIV-1) | p66 RT |
| 2 | B (B) | p51 RT | polymer | 437 | 51120.6 | 1 | UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) | Human immunodeficiency virus type 1 (HIV-1) | |
| 3 | C (C) | monoclonal antibody, heavy chain | polymer | 211 | 23362.7 | 1 | MUS MUSCULUS (MOUSE) | ||
| 4 | D (D) | monoclonal antibody, light chain | polymer | 225 | 24000.8 | 1 | MUS MUSCULUS (MOUSE) | ||
| 5 | E (E) | DNA (5'-D(*AP*T*GP*CP*TP*AP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*GP*TP*G)-3') | polymer | 27 | 8367.4 | 1 | |||
| 6 | F (F) | DNA (5'-D(*AP*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*(MRG)P*CP*GP*CP*CP*(ATM))-3') | polymer | 21 | 6490.3 | 1 | |||
| 7 | G (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 8 | H, I, J, K, L... (A, B, C, D, E...) | water | water | 18.0 | 138 | Chemie (HOH) |
Sequence modifications
A: 1 - 562 (UniProt: P03366)
B: 1 - 428 (UniProt: P03366)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Val 0 | - | expression tag |
| Leu 41 | Met 640 | engineered mutation |
| Asn 67 | Asp 666 | engineered mutation |
| Arg 70 | Lys 669 | engineered mutation |
| Tyr 215 | Thr 814 | engineered mutation |
| Gln 219 | Lys 818 | engineered mutation |
| Cys 258 | Gln 857 | engineered mutation |
| Ser 280 | Cys 879 | engineered mutation |
| Val 561 | Phe 1160 | SEE REMARK 999 |
| PDB | External Database | Details |
|---|---|---|
| Ser 280 | Cys 879 | engineered mutation |
| Gly 429 | - | expression tag |
| Gly 430 | - | expression tag |
| His 431 | - | expression tag |
| His 432 | - | expression tag |
| His 433 | - | expression tag |
| His 434 | - | expression tag |
| His 435 | - | expression tag |
| His 436 | - | expression tag |
| His 437 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 178330.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 178354.6 |
