3KLG

Crystal structure of AZT-resistant HIV-1 Reverse Transcriptase crosslinked to pre-translocation AZTMP-Terminated DNA (COMPLEX N)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Reverse transcriptase/ribonuclease H	polymer	562	64762.3	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	Human immunodeficiency virus type 1 (HIV-1)	p66 RT
2	B, F (B, F)	p51 RT	polymer	437	51120.6	2	UniProt (P03366) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815)	Human immunodeficiency virus type 1 (HIV-1)
3	C, G (C, G)	DNA (5'-D(*AP*T*GP*CP*AP*TP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*GP*TP*G)-3')	polymer	27	8367.4	2
4	D, H (D, H)	DNA (5'-D(*A*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*(MRG)P*CP*GP*CP*CP*AP*(ATM))-3')	polymer	22	6803.5	2

Sequence modifications

A, E: 1 - 560 (UniProt: P03366)

PDB	External Database	Details
Met -1	-	expression tag
Val 0	-	expression tag
Leu 41	Met 640	engineered mutation
Asn 67	Asp 666	engineered mutation
Arg 70	Lys 669	engineered mutation
Tyr 215	Thr 814	engineered mutation
Gln 219	Lys 818	engineered mutation
Cys 258	Gln 857	engineered mutation
Ser 280	Cys 879	engineered mutation
Val 559	Ile 1158	SEE REMARK 999

B, F: 1 - 428 (UniProt: P03366)

PDB	External Database	Details
Ser 280	Cys 879	engineered mutation
Gly 429	-	expression tag
Gly 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	262107.5
All*	Total formula weight	262107.5

*Water molecules are not included.