3KK3

HIV-1 reverse transcriptase-DNA complex with GS-9148 terminated primer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Reverse transcriptase p66 subunit	polymer	560	64521.9	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075)	Human immunodeficiency virus type 1 (HIV-1)	p66 RT
2	B (B)	Reverse transcriptase p51 subunit	polymer	452	52748.4	1	UniProt (P04585) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815)	Human immunodeficiency virus type 1 (HIV-1)	p51 RT
3	C (P)	5'-D(*AP*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*GP*CP*GP*CP*C*(URT))-3'	polymer	21	6400.1	1
4	D (T)	5'-D(*AP*TP*GP*GP*TP*TP*GP*GP*CP*GP*CP*CP*CP*GP*AP*AP*CP*AP*GP*GP*GP*AP*CP*TP*GP*TP*G)-3'	polymer	27	8398.4	1
5	E (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6	F, G (P, T)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	H, I, J, K (A, B, P, T)	water	water		18.0	22	Chemie (HOH)

Sequence modifications

A: 1 - 560 (UniProt: P04585)

PDB	External Database	Details
Cys 258	Gln 845	engineered mutation
Ser 280	Cys 867	engineered mutation

B: 1 - 440 (UniProt: P04585)

PDB	External Database	Details
Met -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Ser 280	Cys 867	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	132068.8
Non-Polymers*	Number of molecules	3
	Total formula weight	216.4
All*	Total formula weight	132285.2

*Water molecules are not included.