3KEG
X-ray Crystallographic Structure of a Y131F mutant of Pseudomonas Aeruginosa Azoreductase in complex with Methyl RED
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | FMN-dependent NADH-azoreductase 1 | polymer | 212 | 23061.1 | 2 | UniProt (Q9I5F3) Pfam (PF02525) | Pseudomonas aeruginosa | FMN-dependent NADH-azo compound oxidoreductase 1, Azo-dye reductase 1 |
| 2 | C, G (A, B) | FLAVIN MONONUCLEOTIDE | non-polymer | 456.3 | 2 | Chemie (FMN) | |||
| 3 | D, E (A) | 2-(4-DIMETHYLAMINOPHENYL)DIAZENYLBENZOIC ACID | non-polymer | 269.3 | 2 | Chemie (MRE) | |||
| 4 | F (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 5 | H, I (A, B) | water | water | 18.0 | 269 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 212 (UniProt: Q9I5F3)
| PDB | External Database | Details |
|---|---|---|
| Phe 131 | Tyr 131 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 46122.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1543.4 | |
| All* | Total formula weight | 47665.5 |
