3K41

Crystal structure of sCD-MPR mutant E19Q/K137M bound to Man-6-P

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Cation-dependent mannose-6-phosphate receptor	polymer	160	18273.5	2	UniProt (P11456) Pfam (PF02157) In PDB	Bos taurus (bovine cow domestic cattle domestic cow)	CD Man-6-P receptor, CD-MPR, 46 kDa mannose 6-phosphate receptor, MPR 46
2	C	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G22573RC)
3	D	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	A, B	6-O-phosphono-beta-D-mannopyranose	non-polymer		260.1	2	Chemie (M6D)
5		water	water		18.0	161	Chemie (HOH)

Sequence modifications

A, B: 1 - 154 (UniProt: P11456)

PDB	External Database	Details
Gln 19	Glu 47	engineered mutation
Gln 31	Asn 59	engineered mutation
Gln 57	Asn 85	engineered mutation
Gln 68	Asn 96	engineered mutation
Gln 87	Asn 115	engineered mutation
Met 137	Lys 165	engineered mutation
His 155	-	expression tag
His 156	-	expression tag
His 157	-	expression tag
His 158	-	expression tag
His 159	-	expression tag
His 160	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	36547.0
Branched	Number of molecules	2
	Total formula weight	1173.1
Non-Polymers*	Number of molecules	2
	Total formula weight	520.3
All*	Total formula weight	38240.3

*Water molecules are not included.