3K3T

E185A mutant of peptidoglycan hydrolase from Sphingomonas sp. A1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Peptidoglycan hydrolase FlgJ	polymer	170	18669.8	1	UniProt (B7XH69) Pfam (PF01832) In PDB	Sphingomonas sp. A1
2	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3		water	water		18.0	123	Chemie (HOH)

Sequence modifications

A: 152 - 313 (UniProt: B7XH69)

PDB	External Database	Details
Met 152	Thr 152	engineered mutation
Ala 153	Pro 153	engineered mutation
Ala 185	Glu 185	engineered mutation
Leu 314	-	expression tag
Glu 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
His 320	-	expression tag
His 321	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	18669.8
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	18861.9

*Water molecules are not included.