3K02

Crystal structures of the GacH receptor of Streptomyces glaucescens GLA.O in the unliganded form and in complex with acarbose and an acarbose homolog. Comparison with acarbose-loaded maltose binding protein of Salmonella typhimurium.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Acarbose/maltose binding protein GacH	polymer	409	43957.9	1	UniProt (B0B0V1) Pfam (PF01547)	Streptomyces glaucescens
2	B (A)	4,6-dideoxy-4-{[(1S,2R,3R,4S,5S)-2,3,4-trihydroxy-5-(hydroxymethyl)cyclohexyl]amino}-alpha-D-allopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranosyl-(1->4)-alpha-D-glucopyranose	non-polymer		809.8	1	Chemie (TXT)
3	C (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
4	D (A)	water	water		18.0	390	Chemie (HOH)

Sequence modifications

A: 17 - 404 (UniProt: B0B0V1)

PDB	External Database	Details
Met -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
Ser 6	-	expression tag
Ser 7	-	expression tag
Gly 8	-	expression tag
Leu 9	-	expression tag
Val 10	-	expression tag
Pro 11	-	expression tag
Arg 12	-	expression tag
Gly 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
Met 16	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	43957.9
Non-Polymers*	Number of molecules	2
	Total formula weight	905.8
All*	Total formula weight	44863.8

*Water molecules are not included.