3JYI
Structural and biochemical evidence that a TEM-1 {beta}-lactamase Asn170Gly active site mutant acts via substrate-assisted catalysis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Beta-lactamase TEM | polymer | 263 | 28884.9 | 6 | UniProt (P62593) Pfam (PF00144) Pfam (PF13354) | Escherichia coli | TEM-1, TEM-2, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2, TEM-16/CAZ-7, TEM-24/CAZ-6, IRT-4, Penicillinase |
| 2 | G, I, K, M, N... (A, B, C, D, E...) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 6 | Chemie (EPE) | |||
| 3 | H, J, L, O (A, B, C, E) | PHOSPHATE ION | non-polymer | 95.0 | 4 | Chemie (PO4) | |||
| 4 | Q, R, S, T, U... (A, B, C, D, E...) | water | water | 18.0 | 174 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 26 - 288 (UniProt: P62593)
| PDB | External Database | Details |
|---|---|---|
| Gly 170 | Asn 168 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 173309.7 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 1809.7 | |
| All* | Total formula weight | 175119.4 |
