3JXS

Crystal structure of XG34, an evolved xyloglucan binding CBM

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Xylanase	polymer	174	18905.7	3	UniProt (Q7WTN6) Pfam (PF02018) In PDB	Rhodothermus marinus (Rhodothermus obamensis)
2	A, B, C	CALCIUM ION	non-polymer		40.1	5	Chemie (CA)
3	B, C	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
4		water	water		18.0	368	Chemie (HOH)

Sequence modifications

A, B, C: 2 - 166 (UniProt: Q7WTN6)

PDB	External Database	Details
Met 1	-	initiating methionine
Tyr 69	Trp 279	engineered mutation
His 72	Glu 282	engineered mutation
Leu 76	Phe 286	engineered mutation
His 110	Phe 320	engineered mutation
Asp 111	Gln 321	engineered mutation
Asp 112	Glu 322	engineered mutation
Phe 149	Tyr 359	engineered mutation
Leu 167	-	expression tag
Glu 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	56717.0
Non-Polymers*	Number of molecules	7
	Total formula weight	318.5
All*	Total formula weight	57035.5

*Water molecules are not included.