3JTR
Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Glutaryl 7-aminocephalosporanic acid acylase | polymer | 169 | 18475.2 | 1 | UniProt (A4ZVL3) In PDB | Pseudomonas sp. | |
2 | B | Glutaryl 7-aminocephalosporanic acid acylase | polymer | 528 | 59168.8 | 1 | UniProt (A4ZVL3) Pfam (PF01804) In PDB | Pseudomonas sp. | |
3 | B | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
4 | water | water | 18.0 | 255 | Chemie (HOH) |
Sequence modifications
A: 1 - 169 (UniProt: A4ZVL3)
B: 1 - 522 (UniProt: A4ZVL3)
PDB | External Database | Details |
---|---|---|
Ser 17 | Thr 46 | SEE REMARK 999 |
His 50 | Gln 79 | SEE REMARK 999 |
Pro 168 | Gln 197 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Arg 100 | Pro 298 | SEE REMARK 999 |
His 523 | - | expression tag |
His 524 | - | expression tag |
His 525 | - | expression tag |
His 526 | - | expression tag |
His 527 | - | expression tag |
His 528 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 77644.0 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 92.1 | |
All* | Total formula weight | 77736.1 |