3JTR

Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glutaryl 7-aminocephalosporanic acid acylase	polymer	169	18475.2	1	UniProt (A4ZVL3)	Pseudomonas sp.
2	B (B)	Glutaryl 7-aminocephalosporanic acid acylase	polymer	528	59168.8	1	UniProt (A4ZVL3) Pfam (PF01804)	Pseudomonas sp.
3	C (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	D, E (A, B)	water	water		18.0	255	Chemie (HOH)

Sequence modifications

A: 1 - 169 (UniProt: A4ZVL3)

PDB	External Database	Details
Ser 17	Thr 46	SEE REMARK 999
His 50	Gln 79	SEE REMARK 999
Pro 168	Gln 197	engineered mutation

B: 1 - 522 (UniProt: A4ZVL3)

PDB	External Database	Details
Arg 100	Pro 298	SEE REMARK 999
His 523	-	expression tag
His 524	-	expression tag
His 525	-	expression tag
His 526	-	expression tag
His 527	-	expression tag
His 528	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	77644.0
Non-Polymers*	Number of molecules	1
	Total formula weight	92.1
All*	Total formula weight	77736.1

*Water molecules are not included.