3JTQ

Mutations in Cephalosporin Acylase Affecting Stability and Autoproteolysis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glutaryl 7-aminocephalosporanic acid acylase	polymer	169	18506.2	1	UniProt (A4ZVL3)	Pseudomonas sp.
2	B (B)	Glutaryl 7-aminocephalosporanic acid acylase	polymer	528	59169.8	1	UniProt (A4ZVL3)	Pseudomonas sp.
3	C, D (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	E, F (A, B)	water	water		18.0	350	Chemie (HOH)

Sequence modifications

A: 1 - 169 (UniProt: A4ZVL3)

PDB	External Database	Details
Ser 17	Thr 46	SEE REMARK 999
His 50	Gln 79	SEE REMARK 999

B: 1 - 522 (UniProt: A4ZVL3)

PDB	External Database	Details
Arg 100	Pro 298	SEE REMARK 999
Asn 210	Leu 408	engineered mutation
His 523	-	expression tag
His 524	-	expression tag
His 525	-	expression tag
His 526	-	expression tag
His 527	-	expression tag
His 528	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	77676.0
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	77860.2

*Water molecules are not included.