3JBY

Cryo-electron microscopy structure of RAG Paired Complex (C2 symmetry)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	V(D)J recombination-activating protein 1	polymer	764	87684.1	2	UniProt (O13033) Pfam (PF00097) Pfam (PF12940) In PDB	Danio rerio (zebra fish)	RAG-1, Endonuclease RAG1, E3 ubiquitin-protein ligase RAG1
2	B, D	V(D)J recombination-activating protein 2	polymer	533	59435.9	2	UniProt (Q1RLW7) Pfam (PF03089) Pfam (PF13341) UniProt (by SIFTS) (O13034) In PDB	Danio rerio (zebra fish)	RAG-2
3	E, H	5'-D(P*CP*AP*CP*AP*GP*TP*GP*CP*TP*AP*CP*AP*GP*AP*C)-3'	polymer	15	4563.0	2		Homo sapiens
4	F, G	RSS intermediate reverse strand	polymer	31	9577.2	2		Homo sapiens
5	I, J	'-D(P*GP*AP*TP*CP*TP*GP*GP*CP*CP*TP*GP*TP*CP*TP*TP*A)-3'	polymer	16	4880.2	2		Homo sapiens
6	A, C	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
7	A, C	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)

Sequence modifications

A, C: 271 - 1031 (UniProt: O13033)

PDB	External Database	Details
Gly 268	-	expression tag
Gly 269	-	expression tag
Ser 270	-	expression tag

B, D: 1 - 530 (UniProt: Q1RLW7)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	332280.8
Non-Polymers*	Number of molecules	6
	Total formula weight	291.1
All*	Total formula weight	332571.9

*Water molecules are not included.