3J2U

Kinesin-13 KLP10A HD in complex with CS-tubulin and a microtubule

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	K	Kinesin-like protein Klp10A	polymer	374	41810.9	1	UniProt (Q960Z0) Pfam (PF00225) In PDB	Drosophila melanogaster (Fruit fly)	Kinesin-like protein at cytological position 10A
2	A, C	Tubulin alpha-1A chain	polymer	451	50107.2	2	UniProt (P81947) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (bovine)	Alpha-tubulin 1, Tubulin alpha-1 chain
3	B, D	Tubulin beta-2B chain	polymer	445	49907.8	2	UniProt (Q6B856) Pfam (PF00091) Pfam (PF03953) In PDB	Bos taurus (bovine)

Sequence modifications

K: 279 - 615 (UniProt: Q960Z0)

PDB	External Database	Details
Met 242	-	expression tag
Arg 243	-	expression tag
Gly 244	-	expression tag
Ser 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag
Gly 252	-	expression tag
Met 253	-	expression tag
Ala 254	-	expression tag
Ser 255	-	expression tag
Met 256	-	expression tag
Thr 257	-	expression tag
Gly 258	-	expression tag
Gly 259	-	expression tag
Gln 260	-	expression tag
Gln 261	-	expression tag
Met 262	-	expression tag
Gly 263	-	expression tag
Arg 264	-	expression tag
Asp 265	-	expression tag
Leu 266	-	expression tag
Tyr 267	-	expression tag
Asp 268	-	expression tag
Asp 269	-	expression tag
Asp 270	-	expression tag
Asp 271	-	expression tag
Lys 272	-	expression tag
Asp 273	-	expression tag
Pro 274	-	expression tag
Ser 275	-	expression tag
Ser 276	-	expression tag
Arg 277	-	expression tag
Ser 278	-	expression tag

A, C: 1 - 451 (UniProt: P81947)

PDB	External Database	Details
Ser 136	Leu 136	conflict
Gly 232	Ser 232	conflict
Gly 265	Ile 265	conflict
Thr 340	Ser 340	conflict
Glu 358	Gln 358	conflict

B, D: 1 - 455 (UniProt: Q6B856)

PDB	External Database	Details
Ala 57	Thr 55	conflict
Val 172	Met 170	conflict
Ala 298	Ser 296	conflict
Val 318	Ile 316	conflict
Gly 450	Glu 440	conflict
Glu 451	Gly 441	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	241840.9
All*	Total formula weight	241840.9

*Water molecules are not included.