3ITV

Crystal structure of Pseudomonas stutzeri L-rhamnose isomerase mutant S329K in complex with D-psicose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	L-rhamnose isomerase	polymer	438	48046.7	4	UniProt (Q75WH8) Pfam (PF01261) In PDB	Pseudomonas stutzeri (Pseudomonas perfectomarina)
2	A, B, C, D	MANGANESE (II) ION	non-polymer		54.9	8	Chemie (MN)
3	A, B, C, D	D-psicose	non-polymer		180.2	4	Chemie (PSJ)
4		water	water		18.0	2101	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 430 (UniProt: Q75WH8)

PDB	External Database	Details
Asn 150	Asp 150	engineered mutation
Lys 329	Ser 329	engineered mutation
Gly 431	-	expression tag
Ser 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	192186.9
Non-Polymers*	Number of molecules	12
	Total formula weight	1160.1
All*	Total formula weight	193347.0

*Water molecules are not included.