3ITO

Crystal structure of Pseudomonas stutzeri L-rhamnose isomerase mutant D327N in complex with D-psicose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	L-rhamnose isomerase	polymer	438	48003.6	4	UniProt (Q75WH8) Pfam (PF01261)	Pseudomonas stutzeri (Pseudomonas perfectomarina)
2	E, F, H, I, K... (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	8	Chemie (MN)
3	G, J, M, P (A, B, C, D)	alpha-D-psicofuranose	non-polymer		180.2	4	Chemie (PSV)
4	Q, R, S, T (A, B, C, D)	water	water		18.0	1436	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 430 (UniProt: Q75WH8)

PDB	External Database	Details
Asn 150	Asp 150	engineered mutation
Asn 327	Asp 327	engineered mutation
Gly 431	-	expression tag
Ser 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag
His 437	-	expression tag
His 438	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	192014.5
Non-Polymers*	Number of molecules	12
	Total formula weight	1160.1
All*	Total formula weight	193174.6

*Water molecules are not included.