3IT3

Crystal Structure Francisella tularensis histidine acid phosphatase D261A mutant complexed with substrate 3'-AMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Acid phosphatase	polymer	342	38558.0	2	UniProt (Q2A612)	Francisella tularensis subsp. holarctica	HISTIDINE ACID PHOSPHATASE
2	C, D (A, B)	[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] dihydrogen phosphate	non-polymer		347.2	2	Chemie (3AM)
3	E, F (A, B)	water	water		18.0	580	Chemie (HOH)

Sequence modifications

A, B: 2 - 336 (UniProt: Q2A612)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 261	Asp 276	engineered mutation
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	77116.0
Non-Polymers*	Number of molecules	2
	Total formula weight	694.4
All*	Total formula weight	77810.4

*Water molecules are not included.