3IHQ

Crystal Structure of Reduced C10S Spx in Complex with the Alpha C-terminal Domain of RNA Polymeras

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Regulatory protein spx	polymer	132	15674.0	1	UniProt (O31602) Pfam (PF03960) In PDB	Bacillus subtilis
2	B	DNA-directed RNA polymerase subunit alpha	polymer	75	8534.9	1	UniProt (P20429) Pfam (PF03118) In PDB	Bacillus subtilis	RNAP subunit alpha, Transcriptase subunit alpha, RNA polymerase subunit alpha
3	A	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
4		water	water		18.0	39	Chemie (HOH)

Sequence modifications

A: 1 - 131 (UniProt: O31602)

PDB	External Database	Details
His 0	-	expression tag
Ser 10	Cys 10	engineered mutation

B: 245 - 314 (UniProt: P20429)

PDB	External Database	Details
Gly 241	-	expression tag
Ser 242	-	expression tag
His 243	-	expression tag
Met 244	-	expression tag
Gly 315	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	24208.8
Non-Polymers*	Number of molecules	1
	Total formula weight	69.1
All*	Total formula weight	24277.9

*Water molecules are not included.