3IFJ

Crystal structure of Mtu recA intein, splicing domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Endonuclease PI-MtuI	polymer	139	15407.5	2	UniProt (P0A5U4) Pfam (PF14890) UniProt (by SIFTS) (P9WHJ3)	Mycobacterium tuberculosis	Protein recA, Recombinase A, Mtu recA intein
2	C, D (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
3	E, F (A, B)	water	water		18.0	197	Chemie (HOH)

Sequence modifications

A, B: 1 - 102 (UniProt: P0A5U4)

PDB	External Database	Details
Val 95	Gln 346	engineered mutation
Arg 96	Pro 347	engineered mutation
Asp 97	Arg 348	engineered mutation
Val 98	Arg 349	engineered mutation
Glu 99	Phe 350	engineered mutation
Thr 100	Asp 351	engineered mutation
Glu 102	Phe 353	engineered mutation

A, B: 404 - 440 (UniProt: P0A5U4)

PDB	External Database	Details
Tyr 421	Phe 672	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	30814.9
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	30945.8

*Water molecules are not included.