3I48

Crystal structure of beta toxin from Staphylococcus aureus F277A, P278A mutant with bound magnesium ions

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Beta-hemolysin	polymer	317	36092.2	2	UniProt (A7LAI8) Pfam (PF03372)	Staphylococcus aureus RN4220
2	C, E (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	D, F (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	G, H (A, B)	water	water		18.0	472	Chemie (HOH)

Sequence modifications

A, B: 2 - 297 (UniProt: A7LAI8)

PDB	External Database	Details
Met -19	-	expression tag
Arg -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Met 1	-	expression tag
Ala 277	Phe 310	engineered mutation
Ala 278	Pro 311	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	72184.3
Non-Polymers*	Number of molecules	4
	Total formula weight	238.6
All*	Total formula weight	72422.9

*Water molecules are not included.