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3I3D

E. COLI (lacZ) BETA-GALACTOSIDASE (M542A) IN COMPLEX WITH IPTG

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A, B, C, DBeta-galactosidasepolymer1023116446.24UniProt (B8LFD6)
Pfam (PF02837)
Pfam (PF00703)
Pfam (PF02836)
Pfam (PF16353)
Pfam (PF02929)
UniProt (by SIFTS) (P00722)
In PDB
Escherichia coli
2A, D, C, B1-methylethyl 1-thio-beta-D-galactopyranosidenon-polymer238.36Chemie (IPT)
3A, D, C, BMAGNESIUM IONnon-polymer24.313Chemie (MG)
4B, C, D, ASODIUM IONnon-polymer23.017Chemie (NA)
5A, B, C, DDIMETHYL SULFOXIDEnon-polymer78.1149Chemie (DMS)
6waterwater18.03533Chemie (HOH)
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: B8LFD6)
PDBExternal DatabaseDetails
Gly 1-expression tag
Ser 2-expression tag
His 3-expression tag
Met 4-expression tag
Leu 5-expression tag
Glu 6-expression tag
Asp 7-expression tag
Pro 8-expression tag
Ala 542Met 543engineered mutation
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight465784.6
Non-Polymers*Number of molecules185
Total formula weight13778.4
All*Total formula weight479563.0
*Water molecules are not included.

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PDB entries from 2024-07-31

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