3I3D
E. COLI (lacZ) BETA-GALACTOSIDASE (M542A) IN COMPLEX WITH IPTG
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Beta-galactosidase | polymer | 1023 | 116446.2 | 4 | UniProt (B8LFD6) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) UniProt (by SIFTS) (P00722) | Escherichia coli | |
| 2 | E, F, SE, TE, UC... (A, D, C, B) | 1-methylethyl 1-thio-beta-D-galactopyranoside | non-polymer | 238.3 | 6 | Chemie (IPT) | |||
| 3 | G, H, I, UE, VC... (A, D, C, B) | MAGNESIUM ION | non-polymer | 24.3 | 13 | Chemie (MG) | |||
| 4 | AB, AD, AF, BB, BD... (B, C, D, A) | SODIUM ION | non-polymer | 23.0 | 17 | Chemie (NA) | |||
| 5 | AA, AC, AE, AG, BA... (A, B, C, D) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 149 | Chemie (DMS) | |||
| 6 | HG, IG, JG, KG (A, B, C, D) | water | water | 18.0 | 3533 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: B8LFD6)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ser 2 | - | expression tag |
| His 3 | - | expression tag |
| Met 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Glu 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Pro 8 | - | expression tag |
| Ala 542 | Met 543 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 465784.6 | |
| Non-Polymers* | Number of molecules | 185 |
| Total formula weight | 13778.4 | |
| All* | Total formula weight | 479563.0 |
