3I3B
E.coli (lacz) Beta-Galactosidase (M542A) in Complex with D-Galactopyranosyl-1-on
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Beta-galactosidase | polymer | 1023 | 116446.2 | 4 | UniProt (B8LFD6) Pfam (PF02837) Pfam (PF00703) Pfam (PF02836) Pfam (PF16353) Pfam (PF02929) UniProt (by SIFTS) (P00722) | Escherichia coli | |
| 2 | E, GC, RA, UD (A, C, B, D) | D-galactonolactone | non-polymer | 178.1 | 4 | Chemie (149) | |||
| 3 | F, G, H, HC, IA... (A, C, B, D) | MAGNESIUM ION | non-polymer | 24.3 | 11 | Chemie (MG) | |||
| 4 | AE, I, J, JC, K... (D, A, C, B) | SODIUM ION | non-polymer | 23.0 | 16 | Chemie (NA) | |||
| 5 | AA, AB, AC, AD, AF... (A, B, C, D) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 126 | Chemie (DMS) | |||
| 6 | FF, GF, HF, IF (A, B, C, D) | water | water | 18.0 | 3208 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 9 - 1023 (UniProt: B8LFD6)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ser 2 | - | expression tag |
| His 3 | - | expression tag |
| Met 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Glu 6 | - | expression tag |
| Asp 7 | - | expression tag |
| Pro 8 | - | expression tag |
| Ala 542 | Met 543 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 465784.6 | |
| Non-Polymers* | Number of molecules | 157 |
| Total formula weight | 11192.5 | |
| All* | Total formula weight | 476977.1 |
