3HZL
Tyr258Phe mutant of NikD, an unusual amino acid oxidase essential for nikkomycin biosynthesis: open form at 1.55A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | NikD protein | polymer | 397 | 44154.5 | 1 | UniProt (Q9X9P9) | Streptomyces tendae | |
| 2 | B (A) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 1 | Chemie (FAD) | |||
| 3 | C (A) | PYRIDINE-2-CARBOXYLIC ACID | non-polymer | 123.1 | 1 | Chemie (6PC) | |||
| 4 | D, E, F (A) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 3 | Chemie (MPD) | |||
| 5 | G (A) | water | water | 18.0 | 416 | Chemie (HOH) |
Sequence modifications
A: 1 - 389 (UniProt: Q9X9P9)
| PDB | External Database | Details |
|---|---|---|
| Phe 258 | Tyr 258 | engineered mutation |
| Leu 390 | - | expression tag |
| Glu 391 | - | expression tag |
| His 392 | - | expression tag |
| His 393 | - | expression tag |
| His 394 | - | expression tag |
| His 395 | - | expression tag |
| His 396 | - | expression tag |
| His 397 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 44154.5 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1263.2 | |
| All* | Total formula weight | 45417.7 |
