3HT3
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus V713P mutant bound to G:dCTP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, D) | DNA polymerase I, large fragment | polymer | 579 | 66055.7 | 2 | Pfam (PF22619) Pfam (PF00476) UniProt (by SIFTS) (D9N168) | Geobacillus stearothermophilus | |
| 2 | C, E (B, E) | 5'-D(*CP*GP*AP*TP*CP*AP*CP*GP*(DOC))-3' | polymer | 9 | 2684.8 | 2 | |||
| 3 | D, F (C, F) | 5'-D(*AP*CP*GP*GP*CP*GP*TP*GP*AP*TP*CP*G)-3' | polymer | 12 | 3703.4 | 2 | |||
| 4 | G, H, I (G, H, I) | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 3 | In PDB BIRD (PRD_900003) | sucrose | ||
| 5 | J, P (A, D) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | K, L, Q (A, D) | 2'-DEOXYCYTIDINE-5'-TRIPHOSPHATE | non-polymer | 467.2 | 3 | Chemie (DCP) | |||
| 7 | M, N, O (D) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 8 | R, S, T, U, V... (A, D, B, C, E...) | water | water | 18.0 | 996 | Chemie (HOH) |
Sequence modifications
A, D: 298 - 876 (PDB: 3HT3)
| PDB | External Database | Details |
|---|---|---|
| Ala 598 | Asp 598 | engineered mutation |
| Pro 713 | Val 713 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 144887.9 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1026.9 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1738.3 | |
| All* | Total formula weight | 147653.0 |
