3HSU

Functional roles of the 6-s-cysteinyl, 8 alpha-N1-histidyl FAD in Glucooligosaccharide Oxidase from Acremonium strictum

Replaces:  3E0T

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Glucooligosaccharide oxidase	polymer	503	55925.4	1	UniProt (Q6PW77) Pfam (PF01565) Pfam (PF08031) In PDB	Acremonium strictum (Black bundle disease fungus)
2	B	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		627.6	1	In PDB BIRD (PRD_900017) GlyTouCan (G47362BJ)		triacetyl-beta-chitotriose
3	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
4	A	FLAVIN-ADENINE DINUCLEOTIDE	non-polymer		785.5	1	Chemie (FAD)
5	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6		water	water		18.0	423	Chemie (HOH)

Sequence modifications

A: 1 - 474 (UniProt: Q6PW77)

PDB	External Database	Details
Glu -5	-	expression tag
Ala -4	-	expression tag
Glu -3	-	expression tag
Ala -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Ala 130	Cys 155	engineered mutation
Tyr 475	-	expression tag
Leu 476	-	expression tag
Glu 477	-	expression tag
Gln 478	-	expression tag
Lys 479	-	expression tag
Leu 480	-	expression tag
Ile 481	-	expression tag
Ser 482	-	expression tag
Glu 483	-	expression tag
Glu 484	-	expression tag
Asp 485	-	expression tag
Leu 486	-	expression tag
Asn 487	-	expression tag
Ser 488	-	expression tag
Ala 489	-	expression tag
Val 490	-	expression tag
Asp 491	-	expression tag
His 492	-	expression tag
His 493	-	expression tag
His 494	-	expression tag
His 495	-	expression tag
His 496	-	expression tag
His 497	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55925.4
Branched	Number of molecules	1
	Total formula weight	627.6
Non-Polymers*	Number of molecules	3
	Total formula weight	1072.2
All*	Total formula weight	57625.1

*Water molecules are not included.