3HPO
Crystal structure of fragment DNA polymerase I from Bacillus stearothermophilus Y714S mutant bound to G:T mismatch
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase I, large fragment | polymer | 580 | 66128.9 | 1 | Pfam (PF22619) Pfam (PF00476) UniProt (by SIFTS) (D9N168) | Geobacillus stearothermophilus | |
| 2 | B (B) | 5'-D(*CP*GP*AP*TP*CP*AP*CP*GP*(DOC))-3' | polymer | 9 | 2684.8 | 1 | |||
| 3 | C (C) | 5'-D(P*GP*GP*CP*GP*TP*GP*AP*TP*CP*G)-3' | polymer | 10 | 3101.0 | 1 | |||
| 4 | D (D) | beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900003) | sucrose | ||
| 5 | E (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | F (A) | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 1 | Chemie (TTP) | |||
| 7 | G (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 8 | H, I, J (A, B, C) | water | water | 18.0 | 724 | Chemie (HOH) |
Sequence modifications
A: 297 - 876 (PDB: 3HPO)
| PDB | External Database | Details |
|---|---|---|
| Ala 329 | Asp 329 | engineered mutation |
| Ser 714 | Tyr 714 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 71914.7 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 602.5 | |
| All* | Total formula weight | 72859.5 |
