3HPI

Crystal structure of maltose-binding protein mutant with bound sucrose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Maltose-binding periplasmic protein	polymer	372	40908.4	2	UniProt (P0AEX9) Pfam (PF01547) In PDB	Escherichia coli	MMBP, Maltodextrin-binding protein
2	C, D	beta-D-fructofuranose-(2-1)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900003)		sucrose
3	A, B	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
4	A, B	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
5		water	water		18.0	202	Chemie (HOH)

Sequence modifications

A, B: 1 - 370 (UniProt: P0AEX9)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Leu 14	Asp 40	engineered mutation
Phe 15	Lys 41	engineered mutation
Tyr 62	Trp 88	engineered mutation
Tyr 111	Glu 137	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81816.7
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	8
	Total formula weight	510.5
All*	Total formula weight	83011.9

*Water molecules are not included.