3HJF
Crystal structure of T. thermophilus Argonaute E546 mutant protein complexed with DNA guide strand and 15-nt RNA target strand
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Argonaute | polymer | 685 | 76742.8 | 1 | UniProt (Q746M7) Pfam (PF22472) Pfam (PF18309) Pfam (PF22474) | Thermus thermophilus | |
| 2 | B (X) | 5'-D(P*TP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*TP*AP*GP*T)-3' | polymer | 21 | 6588.3 | 1 | |||
| 3 | C (Y) | 5'-R(*CP*AP*AP*CP*CP*UP*AP*CP*UP*AP*CP*CP*UP*CP*G)-3' | polymer | 15 | 4671.8 | 1 | |||
| 4 | D (X) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E, F (A, Y) | water | water | 18.0 | 9 | Chemie (HOH) |
Sequence modifications
A: 1 - 685 (UniProt: Q746M7)
| PDB | External Database | Details |
|---|---|---|
| Glu 546 | Asp 546 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 88002.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 24.3 | |
| All* | Total formula weight | 88027.2 |
