3HIQ

Crystal structure of Saporin-L1 mutant (Y73A) from Saponaria officinalis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Vacuolar saporin	polymer	345	38428.6	2	UniProt (Q2QEH4) Pfam (PF00161) In PDB	Saponaria officinalis (Common soapwort)
2		water	water		18.0	190	Chemie (HOH)

Sequence modifications

A, B: 1 - 259 (UniProt: Q2QEH4)

PDB	External Database	Details
Met -85	-	expression tag
Arg -84	-	expression tag
Gly -83	-	expression tag
Ser -82	-	expression tag
His -81	-	expression tag
His -80	-	expression tag
His -79	-	expression tag
His -78	-	expression tag
His -77	-	expression tag
His -76	-	expression tag
Gly -75	-	expression tag
Met -74	-	expression tag
Ala -73	-	expression tag
Ser -72	-	expression tag
Met -71	-	expression tag
Thr -70	-	expression tag
Gly -69	-	expression tag
Gly -68	-	expression tag
Gln -67	-	expression tag
Gln -66	-	expression tag
Met -65	-	expression tag
Gly -64	-	expression tag
Asp -63	-	expression tag
Leu -62	-	expression tag
Tyr -61	-	expression tag
Asp -60	-	expression tag
Asp -59	-	expression tag
Asp -58	-	expression tag
Lys -57	-	expression tag
Asp -56	-	expression tag
His -55	-	expression tag
Pro -54	-	expression tag
Phe -53	-	expression tag
Thr -52	-	expression tag
Val -51	-	expression tag
Ile -50	-	expression tag
Ile -49	-	expression tag
Tyr -48	-	expression tag
Glu -47	-	expression tag
Leu -46	-	expression tag
Asn -45	-	expression tag
Leu -44	-	expression tag
Gln -43	-	expression tag
Gly -42	-	expression tag
Thr -41	-	expression tag
Thr -40	-	expression tag
Lys -39	-	expression tag
Ala -38	-	expression tag
Gln -37	-	expression tag
Tyr -36	-	expression tag
Ser -35	-	expression tag
Thr -34	-	expression tag
Phe -33	-	expression tag
Leu -32	-	expression tag
Lys -31	-	expression tag
Gln -30	-	expression tag
Leu -29	-	expression tag
Arg -28	-	expression tag
Asp -27	-	expression tag
Asp -26	-	expression tag
Ile -25	-	expression tag
Lys -24	-	expression tag
Asp -23	-	expression tag
Pro -22	-	expression tag
Asn -21	-	expression tag
Leu -20	-	expression tag
His -19	-	expression tag
Tyr -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Thr -15	-	expression tag
Asn -14	-	expression tag
Leu -13	-	expression tag
Pro -12	-	expression tag
Val -11	-	expression tag
Ile -10	-	expression tag
Lys -9	-	expression tag
Arg -8	-	expression tag
Pro -7	-	expression tag
Val -6	-	expression tag
Gly -5	-	expression tag
Pro -4	-	expression tag
Pro -3	-	expression tag
Lys -2	-	expression tag
Phe -1	-	expression tag
Thr 0	-	expression tag
Ala 73	Tyr 94	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76857.3
All*	Total formula weight	76857.3

*Water molecules are not included.