3HHT
A mutant of the nitrile hydratase from Geobacillus pallidus having enhanced thermostability
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Nitrile hydratase alpha subunit | polymer | 216 | 24674.2 | 1 | UniProt (Q84FS5) | Geobacillus pallidus | |
| 2 | B (B) | Nitrile hydratase beta subunit | polymer | 229 | 26455.0 | 1 | UniProt (Q84FS6) | Geobacillus pallidus | |
| 3 | C (A) | COBALT (III) ION | non-polymer | 58.9 | 1 | Chemie (3CO) | |||
| 4 | D, E (A, B) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 5 | F, G (A, B) | water | water | 18.0 | 519 | Chemie (HOH) |
Sequence modifications
A: 1 - 216 (UniProt: Q84FS5)
B: 1 - 229 (UniProt: Q84FS6)
| PDB | External Database | Details |
|---|---|---|
| Gly 4 | Asp 4 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Leu 36 | Phe 36 | engineered mutation |
| Ser 103 | Leu 103 | engineered mutation |
| Asn 127 | Tyr 127 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 51129.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 129.8 | |
| All* | Total formula weight | 51259.0 |
