3HAY

Crystal structure of a substrate-bound full H/ACA RNP from Pyrococcus furiosus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Probable tRNA pseudouridine synthase B	polymer	346	39453.9	1	UniProt (Q7LWY0) Pfam (PF08068) Pfam (PF01509) Pfam (PF16198) Pfam (PF01472)	Pyrococcus furiosus	tRNA pseudouridine 55 synthase, Psi55 synthase, tRNA-uridine isomerase, tRNA pseudouridylate synthase
2	B (B)	Small nucleolar rnp gar1-like protein	polymer	104	12340.8	1	UniProt (Q8U029) Pfam (PF04410)	Pyrococcus furiosus	Gar1
3	C (C)	Ribosome biogenesis protein Nop10	polymer	60	7214.6	1	UniProt (Q8U1R4) Pfam (PF04135)	Pyrococcus furiosus
4	D (D)	50S ribosomal protein L7Ae	polymer	130	14314.6	1	UniProt (Q8U160) Pfam (PF01248)	Pyrococcus furiosus
5	E (E)	H/ACA RNA	polymer	71	22949.6	1
6	F (F)	5'-R(*AP*UP*AP*AP*UP*UP*(FHU)P*GP*AP*CP*UP*CP*AP*A)-3'	polymer	14	4452.7	1
7	G (C)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

A: 4 - 343 (UniProt: Q7LWY0)

PDB	External Database	Details
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag
His 349	-	expression tag

C: 1 - 60 (UniProt: Q8U1R4)

PDB	External Database	Details
Lys 2	Arg 2	engineered mutation

D: 3 - 124 (UniProt: Q8U160)

PDB	External Database	Details
Met 1	-	expression tag
Ala 2	-	expression tag
His 125	-	expression tag
His 126	-	expression tag
His 127	-	expression tag
His 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	100726.3
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	100791.7

*Water molecules are not included.