3H97

Structure of a mutant methionyl-tRNA synthetase with modified specificity

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Methionyl-tRNA synthetase	polymer	560	63907.2	1	UniProt (P00959) Pfam (PF09334) Pfam (PF19303) In PDB	Escherichia coli	Methionine-tRNA ligase, MetRS
2	A	CITRIC ACID	non-polymer		192.1	2	Chemie (CIT)
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4		water	water		18.0	983	Chemie (HOH)

Sequence modifications

A: 0 - 547 (UniProt: P00959)

PDB	External Database	Details
Met -12	-	expression tag
Arg -11	-	expression tag
Gly -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 13	Leu 14	engineered mutation
Leu 260	Tyr 261	engineered mutation
Leu 301	His 302	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	63907.2
Non-Polymers*	Number of molecules	3
	Total formula weight	449.7
All*	Total formula weight	64356.9

*Water molecules are not included.