3H8X
Structure determination of DNA methylation lesions N1-meA and N3-meC in duplex DNA using a cross-linked host-guest system
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2 | polymer | 209 | 23706.1 | 1 | UniProt (Q6NS38) Pfam (PF13532) In PDB | Homo sapiens (human) | Alkylated DNA repair protein alkB homolog 2, Oxy DC1 |
| 2 | B | 5'-D(*CP*TP*GP*TP*(ME6)P*TP*(2YR)P*AP*TP*TP*GP*CP*G)-3' | polymer | 13 | 4008.7 | 1 | synthetic construct | ||
| 3 | C | 5'-D(*TP*CP*GP*CP*AP*AP*TP*AP*AP*GP*AP*CP*A)-3' | polymer | 13 | 3968.6 | 1 | synthetic construct | ||
| 4 | water | water | 18.0 | 177 | Chemie (HOH) |
Sequence modifications
A: 56 - 261 (UniProt: Q6NS38)
| PDB | External Database | Details |
|---|---|---|
| Gly 53 | - | expression tag |
| Ser 54 | - | expression tag |
| His 55 | - | expression tag |
| Ser 67 | Cys 67 | engineered mutation |
| Ser 165 | Cys 165 | engineered mutation |
| Cys 175 | Glu 175 | engineered mutation |
| Ser 192 | Cys 192 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 31683.4 | |
| All* | Total formula weight | 31683.4 |
